A Mitochondrion-Specific Photoactivatable Fluorescence Turn-On AIE-Based Bioprobe for Localization Super-Resolution...

Area of Science:

• Biochemistry
• Cell Biology
• Microscopy

Background:

• Mitochondria are vital organelles involved in cellular energy production and signaling.
• Investigating mitochondrial dynamics requires advanced imaging techniques.
• Existing probes may have limitations in specificity or require external activators.

Purpose of the Study:

• To design and synthesize a novel mitochondrion-specific photoactivatable fluorescence turn-on bioprobe.
• To investigate the photoactivation mechanism and cellular behavior of the new bioprobe.
• To apply the bioprobe for super-resolution imaging of mitochondrial dynamics in fixed and live cells.

Main Methods:

• Design and synthesis of the o-TPE-ON+ bioprobe.
• Characterization of the photocyclodehydrogenation photoactivation mechanism.
• Super-resolution microscopy of mitochondria in fixed and live cells.
• Investigation of mitochondrial dynamics under physiological conditions.

Main Results:

• A novel mitochondrion-specific photoactivatable fluorescence turn-on bioprobe (o-TPE-ON+) was successfully prepared.
• The bioprobe operates via a unique photocyclodehydrogenation mechanism.
• o-TPE-ON+ demonstrated unique photoactivation behavior within cells.
• Super-resolution imaging of mitochondria and their dynamics was achieved in both fixed and live cells without external additives.

Conclusions:

• The o-TPE-ON+ bioprobe is a valuable tool for mitochondrion-specific imaging.
• The novel photoactivation mechanism offers advantages for cellular imaging applications.
• This bioprobe facilitates the dynamic investigation of mitochondria in live cells under physiological conditions.