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Updated: Mar 21, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

White-light quantitative phase imaging unit.

YoonSeok Baek, KyeoReh Lee, Jonghee Yoon

|May 4, 2016

Summary

This summary is machine-generated.

We developed a white-light quantitative phase imaging unit (WQPIU) for standard microscopes. This user-friendly tool enables cost-effective, speckle-free phase delay measurements for biological samples.

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Area of Science:

Microscopy
Optical Imaging
Biophysics

Background:

Quantitative Phase Imaging (QPI) offers label-free contrast for biological samples.
Existing QPI methods often require specialized setups or microscope modifications.
There is a need for accessible and user-friendly QPI tools for routine biological research.

Purpose of the Study:

To introduce a practical, stand-alone white-light quantitative phase imaging unit (WQPIU).
To demonstrate the WQPIU's compatibility with standard microscope platforms without modifications.
To showcase the WQPIU's utility for imaging various biological specimens.

Main Methods:

The WQPIU utilizes lateral shearing interferometry and phase shifting interferometry.
It operates under white-light illumination, enabling speckle-free imaging.
The unit is a compact, stand-alone device requiring no additional microscope accessories.

Main Results:

Quantitative phase images of polystyrene beads, human red blood cells, HeLa cells, and mouse white blood cells were successfully acquired.
The WQPIU demonstrated effective measurement of sample-induced phase delay.
Speckle-free imaging was achieved due to white-light illumination.

Conclusions:

The WQPIU provides a cost-effective and user-friendly realization of QPI.
Its compatibility with standard microscopes and speckle-free imaging capabilities are significant advantages.
The WQPIU is poised to broaden the application of QPI in biological sciences as a powerful imaging tool.