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Updated: Mar 21, 2026
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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 1, 2011
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Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological
Chen Xu1, Jinjun Wu, Wenting Liu
1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
Chemical Communications (Cambridge, England)
|May 4, 2016
Abstract:
We present here a novel and efficient method for 5mC detection using a DNA strand exchange reaction (SER) strategy. This enzyme-free method needs no pre-treatment of target DNAs and can be adapted to most of the target duplexes under physiological conditions. The high sequence selectivity of this method can distinguish 5mC from normal cytosine in an accurate manner.
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