Related Experiment Video
Updated: Mar 21, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Comparison of geometrical and diffraction imaging in the space and frequency domains
Abstract:
The geometrical and diffraction point-spread functions of an optical imaging system have been reviewed and compared in the past [Proc. SPIE3729, 434 (1999)PSISDG0277-786X10.1117/12.346821]. In this paper, we review and compare corresponding optical transfer functions. While the truth lies with the diffraction optical-transfer functions (OTF), it is considered easier and quicker to calculate the geometrical OTF, especially for large aberrations. We describe the theory of the two OTFs and explore the range of spatial frequencies and the magnitude of the primary aberrations over which the geometrical OTF may provide a reasonable approximation of the diffraction OTF. Moreover, balancing of spherical aberration with defocus for optimum diffraction OTF is studied as a function of both the aberration value as well as the spatial frequency. How to gauge the progress of an optical design in the frequency domain based on the geometrical OTF is outlined as the ray spot size is used in the space domain.
Related Concept Videos
Interference and Diffraction
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Discrete Fourier Transform
Determination of Crystal Structures
Super-resolution Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology

