Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering NMDA receptor-mediated high firing activity in excitatory human neurons.

iScience·2026

Same author

A neurodevelopmental disorder mutation locks G proteins in the transitory pre-activated state.

Nature communications·2024

Same author

Upregulated GIRK2 Counteracts Ethanol-Induced Changes in Excitability and Respiration in Human Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2024

Same author

5. Collaborative Study on the Genetics of Alcoholism: Functional genomics.

Genes, brain, and behavior·2023

Same author

Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neurons.

bioRxiv : the preprint server for biology·2023

Same author

Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons.

Molecular psychiatry·2022

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Intact glycopeptide characterization using mass spectrometry.

Li Cao¹, Yi Qu², Zhaorui Zhang³

¹a Pharma Research and Development , R&D Platform Technology & Science, GSK , King of Prussia , PA , USA.

Expert Review of Proteomics

|May 4, 2016

Summary

Characterizing intact glycopeptides reveals glycoprotein heterogeneity and biological function. Recent advances in enrichment, separation, mass spectrometry, and bioinformatics enhance intact glycopeptide analysis.

Keywords:

Glycosylation LC-MS/MS bioinformatics glycopeptide post-translational modification proteomics

More Related Videos

Glycopeptide Capture for Cell Surface Proteomics

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Related Experiment Videos

Last Updated: Mar 21, 2026

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Glycopeptide Capture for Cell Surface Proteomics

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Area of Science:

Biochemistry
Proteomics
Glycobiology

Background:

Protein glycosylation is a crucial post-translational modification.
Traditional bottom-up proteomics often misses intact glycopeptide information, limiting understanding of glycoprotein heterogeneity.
Intact glycopeptide analysis is vital for elucidating glycan structure and glycosylation sites, crucial for biological function.

Purpose of the Study:

To review current methods for intact glycopeptide characterization.
To discuss the advantages, limitations, and applications of these methods.
To highlight recent advancements in the field.

Main Methods:

Enrichment techniques for isolating glycopeptides.
Advanced separation strategies for complex mixtures.
Mass spectrometry (MS) for detection and structural analysis.
Bioinformatics tools for data interpretation.

Main Results:

Significant improvements in intact glycopeptide characterization have been achieved.
Current methods offer opportunities to analyze both glycan structure and glycosylation sites simultaneously.
Understanding glycoprotein heterogeneity is now more accessible.

Conclusions:

Intact glycopeptide characterization provides a more comprehensive view of protein glycosylation.
Recent technological advancements facilitate deeper insights into the biological roles of glycosylation.
This review summarizes key methodologies for researchers in the field.