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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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2.52  terahertz dual-axis reflection confocal scanning microscope.

Qi Li, Yi Zhou, Yong-Fa Yang

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |May 4, 2016
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    Summary
    This summary is machine-generated.

    This study introduces a novel dual-axis reflection confocal scanning microscope for terahertz imaging. The system achieves high axial and lateral resolution, demonstrating excellent 3D imaging capabilities for various targets.

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    Area of Science:

    • Optics
    • Terahertz Imaging
    • Microscopy

    Background:

    • Confocal scanning microscopy offers high resolution.
    • Terahertz (THz) imaging provides unique material penetration capabilities.
    • Developing advanced THz microscopy is crucial for various scientific and industrial applications.

    Purpose of the Study:

    • To present a dual-axis reflection confocal scanning microscope operating at 2.52 THz.
    • To evaluate the system's axial and lateral spatial resolution.
    • To demonstrate the 3D imaging capability of the developed microscope.

    Main Methods:

    • The system utilizes a dual-axis reflection confocal scanning design.
    • Axial resolution was measured to be 0.67 mm.
    • Lateral resolution exceeded 0.314 mm, and lengthwise resolution surpassed 0.353 mm.
    • A 0.3 mm pinhole was incorporated to enhance resolution.

    Main Results:

    • The microscope successfully imaged a Chinese character "TAI" on paper and a metal letter "G".
    • Imaging of metal straps and a metal ring verified the system's 3D imaging performance.
    • The system demonstrated satisfying 3D imaging capability for complex structures.

    Conclusions:

    • The developed dual-axis reflection confocal scanning microscope achieves high resolution at 2.52 THz.
    • The system shows significant potential for detailed 3D imaging in various applications.
    • Further advancements in THz microscopy can be achieved with this dual-axis design.