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Ty1 escapes restriction by the self-encoded factor p22 through mutations in capsid
Jessica M Tucker1, David J Garfinkel1
1Department of Biochemistry & Molecular Biology, University of Georgia , Athens, GA, USA.
Mobile Genetic Elements
|May 4, 2016
Summary
The Ty1 retrotransposon
Area of Science:
- Molecular Biology
- Genetics
- Virology
Background:
- Ty1 is a retrotransposon in Saccharomyces, replicating via virus-like particles (VLPs).
- Uncontrolled Ty1 replication can harm the host genome through insertional mutagenesis.
- Ty1 copy number control (CNC) is mediated by the p22 protein.
Purpose of the Study:
- Identify the direct target of p22-mediated inhibition in Ty1 replication.
- Characterize mutations conferring resistance to p22-mediated CNC.
- Elucidate mechanisms of Ty1 copy number control.
Main Methods:
- Analysis of Ty1 Gag protein function and interactions.
- Identification and mapping of CNC-resistant (CNCR) mutations in Gag.
- VLP assembly and p22 incorporation assays.
Main Results:
- Ty1 Gag protein is a direct target of p22 inhibition.
- CNCR mutations in Gag disrupt p22 binding or incorporation into VLPs.
- Mutant Gag proteins that exclude p22 restore Ty1 replication.
Conclusions:
- p22 inhibits Ty1 replication by targeting the Gag protein.
- Specific domains within Gag are crucial for p22 interaction and CNC.
- Ty1 CNC mechanisms share similarities with retroviral capsid-targeting restriction factors.
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