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[CELLULAR COMPOSITION OF THE LAMINA PROPRIA OF JEJUNAL MUCOUS MEMBRANE IN C57BL/6 MICE DURING THE RECOVERY PERIOD
Abstract:
The jejunum of C57 BL/6 mice (n = 5) was examined 7 days after a 30-day-long space flight and in vivarium control animals (n = 6). The cellular composition of the lamina propria of the mucous membranes of the villi and crypt region was studied using histological and morphometric methods. It was found that on Day 7 the recovery of normal cellular composition of the lamina propria was incomplete. In the villi, the number of medium and small lymphocytes, as well as of the plasma cells was reduced. In the crypt region, the changes were less pronounced. In the lamina propria in experimental animals the number of large lymphocytes, macrophages, neutrophils, disintegrating cells and stromal cells was increased. The number of eosinophils was reduced.
Insights
Spaceflight alters mouse jejunum cellularity, with incomplete recovery observed 7 days post-flight. Key immune cells like lymphocytes and plasma cells were reduced, while others like macrophages increased.
Area of Science:
- Gastrointestinal histology
- Spaceflight biology
- Immunology
Context:
- Investigates the impact of space travel on the murine gastrointestinal tract.
- Utilizes C57 BL/6 mice exposed to a 30-day space flight mission.
- Compares space-flown animals to age-matched vivarium controls.
Purpose:
- To analyze the cellular composition of the jejunal lamina propria post-spaceflight.
- To assess the recovery status of the jejunal mucosa 7 days after space exposure.
- To identify specific cell type alterations in the villi and crypt regions.
Summary:
- Histological and morphometric analysis revealed incomplete recovery of jejunal lamina propria cellularity 7 days after space flight.
- A reduction in medium and small lymphocytes and plasma cells was observed in the villi.
- Increased numbers of large lymphocytes, macrophages, neutrophils, and stromal cells were noted in the lamina propria, with reduced eosinophils.
Impact:
- Highlights the persistent cellular changes in the gut mucosa following space missions.
- Provides insights into the immune system's response and recovery dynamics in the gastrointestinal tract after spaceflight.
- Suggests potential implications for astronaut gut health and mission readiness.
