Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CRISPR Learns to Read the Epigenome.

The CRISPR journal·2026

Same author

Transcription factor collaboration enables precise T cell state engineering.

bioRxiv : the preprint server for biology·2026

Same author

Enhancer hubs govern chromatin topology and Th17 cell identity.

bioRxiv : the preprint server for biology·2026

Same author

Reprogramming of neuronal genome function and phenotype by astrocytes.

bioRxiv : the preprint server for biology·2026

Same author

Mismatch tolerance of a gRNA for CRISPR-based gene activation confers broad activity critical for cell reprogramming.

bioRxiv : the preprint server for biology·2026

Same author

dHyperCas12a enables multiplexed CRISPRi screens.

Nature communications·2026

Same journal

Sustainable Refrigerants, Policy Drivers, and Emerging Technologies.

Annual review of chemical and biomolecular engineering·2026

Same journal

Introduction.

Annual review of chemical and biomolecular engineering·2026

Same journal

A Resonant Life.

Annual review of chemical and biomolecular engineering·2026

Same journal

Jamming and Yielding in Dense Suspensions.

Annual review of chemical and biomolecular engineering·2026

Same journal

Beyond Clean: Unraveling Phase Behavior and Rheology of Soaps.

Annual review of chemical and biomolecular engineering·2026

Same journal

The Nonequilibrium Self-Consistent Generalized Langevin Equation Theory of Glasses and Gels.

Annual review of chemical and biomolecular engineering·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Engineering Delivery Vehicles for Genome Editing.

Christopher E Nelson^1,2, Charles A Gersbach^1,2,3

¹Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708.

Annual Review of Chemical and Biomolecular Engineering

|May 6, 2016

Summary

This summary is machine-generated.

Genome engineering offers new gene therapy options, but safe delivery is key. Research is advancing precise genetic modifications for in vivo applications and therapeutic development.

Keywords:

CRISPR TALENs nanoparticle nonviral gene therapy viral gene therapy virus zinc finger nucleases

More Related Videos

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Related Experiment Videos

Last Updated: Mar 21, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Area of Science:

Genetics and Genomics
Biotechnology
Molecular Biology

Background:

Genome engineering technologies have advanced gene therapy possibilities, including disease models, cell therapies, and in vivo gene repair.
Clinical translation of genome engineering faces challenges in developing safe and effective delivery vehicles.
While in vitro applications are established, preclinical research is increasingly focused on in vivo genome editing.

Purpose of the Study:

To review the development of genome engineering platforms.
To discuss the prospects of viral and nonviral delivery vehicles for genome editing.
To highlight promising therapeutic applications of genome engineering.

Main Methods:

Summary of genome engineering platforms: meganucleases, zinc finger nucleases, TALENs, and CRISPR/Cas9.
Review of viral and nonviral delivery systems for genome editing tools.
Discussion of preclinical and clinical advancements in therapeutic applications.

Main Results:

Genome engineering platforms offer flexibility for precise genetic modifications.
Significant progress is being made in developing safe and effective delivery vehicles.
Promising preclinical data supports in vivo applications and therapeutic development.

Conclusions:

Genome engineering holds significant potential for gene therapy.
Overcoming delivery challenges is crucial for clinical translation.
Continued research in delivery systems and therapeutic applications will drive future advancements.