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Related Experiment Video

Updated: Mar 21, 2026

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells
08:46

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Published on: July 22, 2025

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Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components.

Marek Drozdz1, David J Vaux2

  • 1Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2016
PubMed
Summary
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This study introduces novel single-cell pulse-chase techniques for tracking biological processes over time. These methods enable detailed observation of molecular dynamics at the individual cell level, overcoming limitations of traditional ensemble measurements.

Area of Science:

  • Cell biology
  • Biochemistry
  • Molecular dynamics

Background:

  • Traditional pulse-chase methods provide ensemble measurements, limiting insights into single-cell behavior.
  • Understanding dynamic biological processes at the single-cell level is crucial for deciphering cellular heterogeneity and function.

Purpose of the Study:

  • To develop and exemplify novel single-cell level pulse-chase techniques.
  • To enable time-dependent studies of molecular trafficking and protein maturation in individual cells.

Main Methods:

  • Developed three distinct single-cell pulse-chase methodologies: true pulse-chase, photoactivation, and pharmacological induction.
  • Applied these methods to track nuclear envelope targeting of phospholipids and lamin proteins (lamin B1, prelamin A).
Keywords:
Nucleoplasmic reticulumPhotoactivatable fluorescent proteinsPulse-chaseSecondary ion mass spectrometrySingle-cell assaysStable isotope labelingSuper-resolution light microscopy

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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

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Single-Molecule Imaging of Nuclear Transport
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Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

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Related Experiment Videos

Last Updated: Mar 21, 2026

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells
08:46

Using LEXY and LINuS Optogenetics Tools and Automated Image Analysis to Quantify Nucleocytoplasmic Transport Dynamics in Live Cells

Published on: July 22, 2025

792
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
08:59

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

12.0K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.8K

Main Results:

  • Successfully demonstrated the application of single-cell pulse-chase techniques for dynamic molecular tracking.
  • Visualized region-selective nuclear envelope targeting of nascent phospholipids and proteins in real-time within single cells.

Conclusions:

  • The novel single-cell pulse-chase methods significantly advance the study of dynamic biological processes.
  • These techniques offer unprecedented resolution for investigating molecular evolution and trafficking at the individual cell level.