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Published on: April 20, 2017
Furan-PNA: a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA
A Manicardi1, E Gyssels, R Corradini
1Department of Chemistry, University of Parma, Parco Area delle Scienze 17/A, Parma 43124, Italy. alex.manicardi@unipr.it.
Abstract:
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated. The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown.
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