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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Mar 21, 2026

Dissection of 6.5 dpc Mouse Embryos

Dissection of 6.5 dpc Mouse Embryos

Published on: February 25, 2007

Mouse JMJD4 is dispensable for embryogenesis.

Hyunjin Yoo¹, Dabin Son¹, Young Jae Lee²

¹Department of Nanobiomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan-si, Republic of Korea.

Molecular Reproduction and Development

|May 6, 2016

Summary

Jumonji C domain-containing demethylase 4 (JMJD4) plays a role in mRNA translation. However, JMJD4 is dispensable for mouse embryonic development and embryonic stem cell differentiation.

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Last Updated: Mar 21, 2026

Dissection of 6.5 dpc Mouse Embryos

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Published on: February 25, 2007

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

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Published on: June 6, 2016

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Published on: July 10, 2018

Area of Science:

Developmental biology
Epigenetics
Gene regulation

Background:

Jumonji C domain-containing demethylase 4 (JMJD4) is implicated in mRNA translation regulation.
Its specific in vivo function during mouse development remains largely uncharacterized.

Purpose of the Study:

To investigate the role of JMJD4 in embryonic stem cell (ESC) differentiation.
To determine the necessity of JMJD4 during embryonic development using a knockout mouse model.

Main Methods:

Examined JMJD4 contribution to ESC differentiation.
Generated and analyzed Jmjd4-knockout mice for developmental defects.
Assessed ESC morphology and pluripotent gene expression in Jmjd4-null cells.

Main Results:

JMJD4 expression decreased during ESC differentiation and localized to specific embryonic organs.
Jmjd4-null ESCs displayed normal morphology and maintained pluripotency.
Jmjd4-knockout embryos were born at expected Mendelian ratios without apparent developmental abnormalities.

Conclusions:

JMJD4 is not essential for mouse embryonic development.
The demethylase JMJD4 is dispensable for embryonic stem cell differentiation and pluripotency maintenance in vivo.