[Immunoprint technic: a new method for planned production of allergen-specific monoclonal antibodies]

Working on the isolation of allergens from crude pollen extracts we were looking for a simple method to produce monoclonal antibodies (mab) specific for a major allergen of cultivated rye (S. cereale) with an isoelectric point (pl) of 5.9. We used immunization with allergen isolated from nitrocellulose blots. Allergen was obtained by isoelectric focusing (IEF) of a rye pollen extract followed by immunoprint and detection of allergen bound to nitrocellulose by a monoclonal antibody (mab). Fusion with myeloma cell line P3X63Ag8.U1 led to production of 7 different mab specific for the protein with pl 5.9. The method seems to be a simple way to produce mab specific for a certain component of a crude protein mixture as a pollen extract.