Simple, Efficient CRISPR-Cas9-Mediated Gene Editing in Mice: Strategies and Methods

Area of Science:

• Genetics
• Molecular Biology
• Genomics

Background:

• Targeted nucleases enable precise genome editing across diverse species.
• The mouse is an ideal model organism for genetic studies due to its biology and cost-effectiveness.

Purpose of the Study:

• To review strategies for designing single guide RNAs (sgRNAs) for targeted nucleases.
• To detail the practical processes for precision targeting and modification of the mouse genome.
• To outline the establishment of novel precision genetically modified mouse lines.

Main Methods:

• Utilizing targeted nucleases for high-efficiency, rapid, and economical genome modification.
• Employing homologous recombination alongside targeted nucleases for precise genomic tailoring.
• Generating genetically modified mouse models directly in zygotes from any mouse strain.

Main Results:

• Enabling precision nucleotide modification of the mouse genome.
• Facilitating the creation of accurate models for human diseases and conditions.
• Allowing sequential refinement of mouse models to better mimic human pathologies.

Conclusions:

• Targeted nucleases and homologous recombination offer powerful tools for mouse genome engineering.
• These advancements accelerate the development of sophisticated mouse models for disease research.
• Precision genetic modification in mice is crucial for understanding gene function and developing therapeutics.