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Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction

Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction

Published on: November 6, 2019

Germ-Free Mice Model for Studying Host-Microbial Interactions.

Yogesh Bhattarai¹, Purna C Kashyap²

¹Department of Gastroenterology and Hepatology, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 7, 2016

Summary

This summary is machine-generated.

Germ-free (GF) mice are crucial for studying host-microbial interactions in obesity and immunity. This review highlights their importance and provides guidance on establishing gnotobiotic facilities for GF mouse research.

Keywords:

Germ-free Gnotobiotic Gut microbiome

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Area of Science:

Microbiology
Immunology
Physiology

Background:

Germ-free (GF) mice lack a microbiome, making them a unique model for host-microbial interactions.
Understanding these interactions is vital for health and disease research.

Purpose of the Study:

To review the utility of GF mice in studying host-microbial interactions.
To highlight their application in obesity, immune development, and gastrointestinal physiology.
To provide practical guidance on gnotobiotic facility setup and GF status assessment.

Main Methods:

Literature review of studies utilizing GF mice.
Protocol outline for gnotobiotic facility establishment and maintenance.

Main Results:

GF mice are essential for dissecting the specific roles of microbes in various physiological processes.
Current literature supports their application in obesity, immunology, and gut health research.

Conclusions:

GF mice are indispensable for advancing our understanding of host-microbial dynamics.
Establishing and maintaining gnotobiotic facilities is key to leveraging this model system effectively.