Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination

Area of Science:

• Electrochemistry
• Biomaterials Science
• Nanotechnology

Background:

• Enzyme immobilization is crucial for developing stable and sensitive electrochemical biosensors.
• Nitrogen-doped carbon dots (N-CDs) are emerging nanomaterials with unique electronic properties.
• Developing efficient substrates for enzyme immobilization remains a key challenge in biosensor technology.

Purpose of the Study:

• To introduce nitrogen-doped carbon dots as a novel substrate for enzyme immobilization.
• To develop a stable and highly sensitive electrochemical biosensor for glucose detection.
• To investigate the electrocatalytic properties of N-CDs for enhanced biosensor performance.

Main Methods:

• One-step synthesis of N-CDs from polyacrylamide.
• Immobilization of glucose oxidase (GOx) onto N-CDs-modified glassy carbon electrodes via amino-carboxyl reactions.
• Electrochemical detection of glucose in serum samples using the developed biosensor.

Main Results:

• N-CDs provide a stable and effective platform for GOx immobilization.
• The nitrogen-induced charge delocalization in N-CDs enhances electrocatalytic activity.
• The developed biosensor exhibits efficient glucose detection in serum with a detection limit of 0.25 mM (1-12 mM range).
• The biosensor demonstrates good reproducibility, stability, and selectivity under physiological conditions.

Conclusions:

• Nitrogen-doped carbon dots are a promising novel material for constructing robust electrochemical biosensors.
• The developed N-CDs-based biosensor offers a sensitive and selective method for glucose determination.
• This approach highlights the potential of N-CDs in advancing electrochemical sensing technologies.