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Related Experiment Video

Updated: Mar 21, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

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KiT: a MATLAB package for kinetochore tracking.

Jonathan W Armond1, Elina Vladimirou2, Andrew D McAinsh2

  • 1Division of Biomedical Cell Biology, Mechanochemical Cell Biology Building, Warwick Medical School Warwick Systems Biology Centre and Mathematics Institute, University of Warwick, Coventry, CV4 7AL, UK.

Bioinformatics (Oxford, England)
|May 7, 2016
PubMed
Summary

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Single kinetochores execute an ordered series of molecular events as the spindle assembly checkpoint is silenced.

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Bayesian data driven modelling of kinetochore dynamics: Space-time organisation of the human metaphase plate.

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Age-dependent loss of cohesion protection in human oocytes.

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Principles and dynamics of spindle assembly checkpoint signalling.

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The first mitotic division of human embryos is highly error prone.

Nature communications·2022

We developed KiT (Kinetochore Tracking), an open-source software for automated analysis of kinetochore motion during cell division. This tool enhances the study of chromosome dynamics in live-cell imaging.

Area of Science:

  • Cell Biology
  • Biophysics
  • Computational Biology

Background:

  • Kinetochores are crucial protein complexes that mediate chromosome attachment to the mitotic spindle during cell division.
  • Understanding kinetochore dynamics is essential for comprehending chromosome segregation and cell cycle regulation.
  • Automated quantitative tracking of kinetochore motion has revealed complex behaviors previously unobserved.

Purpose of the Study:

  • To introduce KiT (Kinetochore Tracking), a novel, user-friendly, open-source software package.
  • To provide a tool for automated, quantitative tracking of kinetochore motion in live-cell fluorescent microscopy movies.
  • To facilitate detailed analysis of kinetochore behavior during mitosis.

Main Methods:

  • KiT software package implemented in MATLAB.

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Last Updated: Mar 21, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
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  • Supports 2D, 3D, and multi-color live-cell fluorescent movies.
  • Includes features for fluorescence quantification, integrated deconvolution, parallel processing, and multiple particle localization algorithms.

    • Main Results:

    • KiT enables easy-to-use, automated tracking of kinetochores.
    • The software supports diverse imaging modalities and advanced image processing techniques.
    • Facilitates in-depth quantitative analysis of kinetochore movement and behavior.

    Conclusions:

    • KiT is a valuable, freely available resource for researchers studying mitosis and chromosome dynamics.
    • The software simplifies and enhances the quantitative analysis of kinetochore behavior from live-cell imaging data.
    • KiT contributes to advancing our understanding of the intricate mechanics of cell division.