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Glucose Stability Study: NaF/Citrate Plasma Versus Serum.

Xiaolian Zhang, Chengzhi Rong, Haiwei Li

    Clinical Laboratory
    |May 10, 2016
    PubMed
    Summary

    For accurate glucose testing, NaF/citrate plasma tubes are superior to serum tubes for shipping venous whole blood samples at room temperature for up to 10 hours, minimizing glycolysis.

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    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Laboratory Medicine

    Background:

    • Accurate glucose concentration measurements are crucial in clinical diagnostics.
    • Specimen shipment conditions can impact glucose test results.
    • Understanding sample collection method effects on glucose stability is vital.

    Purpose of the Study:

    • To evaluate the impact of sample collection method on glucose measurements.
    • To compare glucose stability in different shipment conditions at room temperature for up to 10 hours.

    Main Methods:

    • Paired fasting venous blood samples were collected in NaF/citrate and serum tubes.
    • Glucose levels were measured at hourly intervals for 10 hours using the Glucose GOD-PAP method.
    • Statistical analysis included Passing-Bablok regression to compare sample types.

    Main Results:

    • Plasma samples (NaF/citrate) showed significantly higher glucose concentrations than serum samples at all time points.
    • Serum tubes exhibited higher glycolysis (13.4%) compared to NaF/citrate tubes (2%) after 10 hours.
    • A negative bias was observed for samples collected in serum tubes.

    Conclusions:

    • NaF/citrate plasma tubes are suitable for shipping venous whole blood samples within 10 hours at room temperature.
    • This method minimizes significant glycolysis, ensuring more accurate glucose measurements.
    • The choice of collection tube significantly impacts glucose stability during transport.