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Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells
Seung Hyuck Bang1, Dong Jun Park1, Yang-Hoon Kim2
1Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
Objectives:
There have been developed to use targeting ability for antimicrobial, anticancerous, gene therapy and cosmetics through analysis of various membrane proteins isolated from cell organelles.
Methods:
It was examined about the lysosomal membrane protein extracted from lysosome isolated from HeLa cell treated by 100 ppm melanin for 24 hours in order to find associated with targeting ability to melanin using by 2-dimensional electrophoresis.
Results:
The result showed 14 up-regulated (1.5-fold) and 13 down-regulated (2.0-fold) spots in relation to melanin exposure.
Conclusions:
It has been found that lysosomal membrane proteins are associated with melanin to decolorize and quantity through cellular activation of lysosome.
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