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Activity-Based Proteome Profiling Probes Based on Woodward's Reagent K with Distinct Target Selectivity
Yong Qian1, Marc Schürmann1, Petra Janning1
1Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, Dortmund, Germany.
Angewandte Chemie (International Ed. in English)
|May 10, 2016
Summary
Woodward's reagent K (WRK) probes selectively label proteins, including macrophage migration inhibitory factor (MIF). These novel probes enable imaging of MIF activity in living cells.
Area of Science:
- Biochemistry
- Chemical Biology
- Proteomics
Background:
- Woodward's reagent K (WRK) is a reactive compound used for nonspecific protein labeling.
- Previous applications targeted nucleophilic amino acids like histidine and cysteine.
Purpose of the Study:
- Develop novel WRK-derived activity-based probes.
- Investigate the selectivity of these probes against the human proteome.
- Explore the utility of WRK probes for studying macrophage migration inhibitory factor (MIF).
Main Methods:
- Synthesis of a panel of WRK-derived activity-based probes.
- Proteomic analysis to determine probe selectivity.
- Cellular imaging experiments using fluorescently labeled probes.
Main Results:
- WRK-derived probes demonstrated unexpected selectivity for a limited number of human proteins.
- Probes exhibited unique reactivity with the N-terminal proline of MIF.
- Successful labeling and imaging of MIF activity in living cells were achieved.
Conclusions:
- WRK-derived probes offer a selective tool for protein labeling.
- These probes provide a novel method for studying MIF and its biological activities.
- The findings open new avenues for activity-based protein profiling.
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