Related Experiment Video
Updated: Mar 21, 2026
The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
Chloroplast based genetic diversity among Chinese grapes genotypes
Tariq Pervaiz1, Cheng Zhang1, Muhammad Faheem2
1a Key Laboratory of Genetics and Fruit Development, College of Horticulture , Nanjing Agriculture University , Nanjing , PR China.
Abstract:
Chloroplast simple sequence repeat (cpSSR) markers previously reported were used to analyze chloroplast diversity of Chinese grapes. Nine cpSSR primer pairs from the chloroplast genomes of citrus and tobacco (Nicotiana tabacum L.) were found useful for analyzing the grapes chloroplast genome (cpDNA). Selected cpSSR primer pairs showed polymorphism among 64 varieties, with polymorphism information content (PIC) values ranging from 0.522 to 0.659, with an average of 0.590. Results depicted that a total number of 43 alleles were detected on nine loci with an average of 4.77 alleles per locus with highest number of six alleles for marker ARCP2 and CCMP10. Gene diversity explained by these cpSSR makers ranged from 4 to 68% with an average of 63.66% per marker. The genetic relationship suggested by UPGMA based dendrogram divided the genotypes into three main clusters and four subgroups, results showed that 2B is the biggest group. Overall Hong Ma Ge and Da LI Mei Gui Xiung 98% similar, while 64-1-19 was the most diverse as compared to rest of genotypes.
More Related Videos
12:33Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
07:12Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
Published on: June 30, 2023
Related Concept Videos
01:25Genetic Variation
Genes exist in different versions called alleles,...
01:20Monohybrid Crosses
02:46Chi-square Analysis
The chi-square test was developed by Pearson in 1990.
The first step of performing a Chi-square analysis is to establish a null hypothesis, which assumes that there is no real...
01:18Dihybrid Crosses
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...