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Updated: Mar 21, 2026
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Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
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Avoiding a second amniocentesis to corroborate prenatal diagnosis by using refrigerated samples.
L A Méndez-Rosado1, E Cuétara2, O Molina-Gamboa1
1a National Center of Medical Genetics of Cuba, Havana Medical University , Havana , Cuba and.
Summary
Refrigerated amniotic fluid (AF) samples are effective for fluorescence in situ hybridization (FISH) prenatal diagnosis. This method accurately identifies chromosomal abnormalities, avoiding the need for additional amniocentesis and reducing patient stress.
Area of Science:
- Prenatal diagnostics
- Cytogenetics
- Molecular biology
Background:
- Prenatal diagnosis of chromosomal abnormalities is crucial for fetal health.
- Conventional methods may require additional procedures, causing stress.
- Fluorescence in situ hybridization (FISH) is a key cytogenetic technique.
Purpose of the Study:
- To evaluate the utility of refrigerated amniotic fluid (AF) for fluorescence in situ hybridization (FISH).
- To determine if refrigerated AF can provide accurate prenatal diagnosis.
- To avoid risks and psychological stress associated with repeat amniocentesis.
Main Methods:
- Non-cultured amniotic fluid (AF) samples were analyzed using FISH.
- Samples were divided into fresh (FCAF) and refrigerated (RSAF) groups.
- Refrigerated samples were stored for up to 18 days.
Main Results:
- No significant differences were observed between fresh and refrigerated AF samples.
- FISH on refrigerated AF successfully corroborated chromosomal mosaicism and pseudomosaicism.
- Accurate fetal sex determination and marker chromosome origin identification were achieved.
Conclusions:
- Refrigerated amniotic fluid (AF) is a viable and effective biological material for FISH analysis.
- FISH on refrigerated AF enables accurate prenatal diagnosis.
- This approach eliminates the need for additional amniocentesis, reducing patient burden.
