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Updated: Mar 21, 2026
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface
Jitendra K Biswal1, Rajeev Ranjan2, Bramhadev Pattnaik2
1ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteswar, Nainital, Uttarakhand, 263138, India. jkubiswal@gmail.com.
Objective:
To determine whether the G-H loop of foot-and-mouth disease virus (FMDV) serotype O can function as a target structure to harbour and display serotype Asia1 antigenic epitope at the surface.
Results:
Using reverse genetics, FMDV serotype O IND R2/1975 displaying a FMDV serotype Asia1 B cell epitope at the capsid surface was constructed. The epitope-inserted recombinant chimeric virus was genetically stable up to ten serial passages in cell culture and exhibited growth properties similar to the parental serotype O virus. Furthermore, the surface-displayed Asia1 epitope able to react with serotype Asia1 specific antibodies in a competitive ELISA. Importantly, the recombinant chimeric virus showed neutralizing activity to both serotype O and Asia1 polyclonal antibodies.
Conclusion:
The capsid protein of FMDV serotype O can effectively display potent epitope of other serotypes, making this an attractive approach for the design of new generation bi-valent FMD vaccines.
