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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
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TRP-Na(+)/Ca(2+) Exchanger Coupling.

Alan G S Harper1, Stewart O Sage2

  • 1Institute for Science and Technology in Medicine, Keele University, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK.

Advances in Experimental Medicine and Biology
|May 11, 2016
PubMed
Summary
This summary is machine-generated.

Na(+)/Ca(2+) exchangers (NCXs) can facilitate calcium entry into non-excitable cells via reverse mode. This process is linked to localized sodium entry through TRPC channels, potentially occurring in nanojunctions.

Keywords:
Calcium entryNa+/Ca2+ exchangersReverse mode NCX activitySodium entryTRPC channels

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Area of Science:

  • Cellular Physiology
  • Ion Transport Mechanisms
  • Membrane Biology

Background:

  • Na(+)/Ca(2+) exchangers (NCXs) traditionally remove Ca(2+) from non-excitable cells.
  • Emerging evidence highlights NCX reverse mode for Ca(2+) entry into these cells.
  • This reverse mode requires altered transmembrane ion gradients and membrane potential.

Purpose of the Study:

  • To review evidence for functional/physical coupling between NCXs and non-selective cation channels.
  • To explore how this coupling influences NCX activity in non-excitable cells.
  • To investigate the role of nanojunctions in triggering NCX reverse mode.

Main Methods:

  • Literature review of studies on NCX and non-selective cation channel interactions.
  • Analysis of evidence for physical and functional coupling.
  • Focus on the role of nanojunctions in ion transport.

Main Results:

  • Evidence supports the coupling of NCXs to non-selective cation channels, including TRPC channels.
  • This coupling is crucial for initiating NCX reverse mode.
  • Nanojunctions may provide the localized environment for Na(+) influx needed for reverse mode.

Conclusions:

  • NCX reverse mode is a significant pathway for Ca(2+) entry in non-excitable cells.
  • Coupling with cation channels, particularly TRPC, is key to this process.
  • Nanojunctions are proposed as critical sites facilitating the ion dynamics for NCX reverse mode.