Related Experiment Video

Updated: Mar 21, 2026

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K

PO-46 - Influence of extracellular vesicles derived from AML patients on stem cells and their microenvironment

I Tzoran1, A Rebibo-Sabbah2, B Brenner2

  • 1Department of Hematology and Bone Marrow Transplantation, Rambam Health Care Campus; Internal Medicine C, Rambam Health Care Campus; Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology; Haifa, Israel.

Thrombosis Research
|May 11, 2016
PubMed
Abstract

Insights

Extracellular vesicles (EVs) from acute myeloid leukemia (AML) blast cells can influence hematopoietic stem cells (HSCs). Mesenchymal stem cells (MSCs) appear crucial for this process, highlighting a novel mechanism in AML progression.

Area of Science:

  • Hematology
  • Cell Biology
  • Cancer Research

Background:

  • Acute myeloid leukemia (AML) is a rapidly progressing cancer characterized by uncontrolled growth of leukemic blast cells.
  • Extracellular vesicles (EVs) are released by cells and carry surface proteins and antigens that reflect their cell of origin, including in pathological conditions like AML.

Purpose of the Study:

  • To investigate if bone marrow EVs from AML patients originate from blast cells.
  • To determine if these AML-derived EVs can affect hematopoietic stem cells (HSCs).
  • To explore this interaction within a co-culture system of HSCs and mesenchymal stem cells (MSCs), simulating a pseudo-natural microenvironment.

Main Methods:

  • Bone marrow samples were collected from AML patients and healthy controls at different disease stages.
  • EVs were characterized for concentration, origin, and protein expression using flow cytometry (FACS).
  • HSCs were isolated from cord blood and co-incubated with AML EVs in the presence or absence of MSCs, with EV internalization assessed by FACS-AMNIS and confocal microscopy. MicroRNA expression (mir-125b, mir-155) was analyzed via RT-PCR.

Main Results:

  • AML patients exhibited higher blast EV counts compared to controls and patients in remission.
  • Internalization of AML-derived EVs (CD117+/CD33+) into HSCs was confirmed, with EVs found within cell cytoplasm and nuclei.
  • Exposure to AML EVs, particularly in the presence of MSCs, showed a tendency to increase mir-125b and mir-155 expression in HSCs.

Conclusions:

  • Bone marrow EVs in AML patients are indeed derived from blast cells.
  • These EVs can influence the differentiation of naive stem cells through internalization.
  • Mesenchymal stem cells appear to play a significant role in mediating the effects of AML EVs on stem cells.

More Related Videos

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

7.4K
Modeling Chemotherapy Resistant Leukemia In Vitro08:41

Modeling Chemotherapy Resistant Leukemia In Vitro

Published on: February 9, 2016

9.7K

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.3K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.9K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.2K
Overview of Exosomes01:36

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.9K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.8K