Related Experiment Video
Updated: Mar 21, 2026
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
The protective effect of North Schisandra Lignans on vascular endothelial cell oxidation injuries
Ruowen Zhang1, Yilin Wang2, Dong Zhu3
1Beihua University Faculty of Medicine, Beihua University, Jilin, China.
Background:
In this study, the authors cultivated ECV-304 in vitro and incubated cells with H2O2, established injury models, and induced oxidized endothelial cell apoptosis. This model makes it possible to choose suitable concentrations of North Schisandra Lignans.
Objective:
To study the protective effects of North Schisandra Lignans on human umbilical vein endothelial cell injuries.
Methods:
Endothelial cell growth and proliferation activity were detected through the MTT method. The colorimetric method was used to determine superoxide dismutase (SOD) activity in the cell culture solution, as well as malondialdehyde (MDA) content in the cell.
Results:
North Schisandra Lignans noticeably decreased ECV-304 cell injury induced by H2O2. Moderate and high concentrations of North Schisandra Lignans could significantly lower MDA content and heighten SOD activity. These differences were significant compared to the H2O2 group (P< 0.05).
Conclusions:
North Schisandra Lignans had an obvious protective effect on ECV-304 injured by H2O2$. The mechanism decreases MDA production and heightened SOD activity.
