Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excitation-regulated fluorescence and triplet yield in 3-nitrobenzanthrone: Experimental and theoretical insights into excited-state dynamics.

The Journal of chemical physics·2026
Same author

Linear and Nonlinear Spectroscopic Insights Into the Synergistic Solvation of Rhodamine-6G in Methanol-Dichloromethane Binary Solvent Mixture.

Journal of fluorescence·2026
Same author

Strain-Induced Long-Lived Heterogeneous Luminescent States in the Outer Layers Unlock Charge Extraction in Stable Graded-Alloy Giant Quantum Dots.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Histoacryl glue injection in walled-off necrosis impression on gastric wall mistaken as gastric varix: an unintended adverse event having therapeutic benefit.

iGIE : innovation, investigation and insights·2026
Same author

Convective Onset in Thermal Lensing: Systematic Pulse-Number-Dependent Experiments and Theoretical Insights into the Femtosecond Regime.

The journal of physical chemistry. B·2026
Same author

Femtosecond-laser-induced optical confinement with ping-pong motion.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Mar 21, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

12.6K

Two-Photon Fluorescence Tracking of Colloidal Clusters.

Debjit Roy1, Dipankar Mondal1, Debabrata Goswami2

  • 1Department of Chemistry, Indian Institute of Technology Kanpur, Uttar Pradesh, 208016, India.

Journal of Fluorescence
|May 12, 2016
PubMed
Summary
This summary is machine-generated.

This study measures optical trap stiffness of nanoparticle clusters in situ using two-photon fluorescence (TPF) tracking. This method tracks colloidal cluster formation without position sensitive detectors.

Keywords:
FluorescenceOptical tweezersTrapping

More Related Videos

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.7K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.4K

Related Experiment Videos

Last Updated: Mar 21, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

12.6K
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.7K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.4K

Area of Science:

  • Colloidal science
  • Nanotechnology
  • Optical physics

Background:

  • In situ dynamics of colloidal cluster formation from nanoparticles remain underexplored.
  • Optical trap stiffness is crucial for understanding optical trap strength.
  • Existing methods may lack precision for in situ nanoparticle aggregate analysis.

Purpose of the Study:

  • To demonstrate in situ measurement of effective 3D optical trap stiffness for nanoparticles and their aggregates.
  • To utilize two-photon fluorescence (TPF) for tracking colloidal cluster formation.
  • To distinguish colloidal clusters from monomers using TPF tracking.

Main Methods:

  • Employed two-photon fluorescence (TPF) for nanoparticle tracking.
  • Developed a method for in situ measurement of optical trap stiffness without position sensitive detectors.
  • Applied TPF to monitor nanosphere colloidal cluster formation in an optical trapping regime.

Main Results:

  • Successfully measured effective 3D optical trap stiffness in situ.
  • TPF demonstrated a zero-background detection scheme with high signal-to-noise ratio.
  • Colloidal clusters were effectively distinguished from monomers via TPF tracking.

Conclusions:

  • TPF is a viable technique for in situ measurement of optical trap stiffness of nanoparticle aggregates.
  • This method advances the study of colloidal cluster dynamics in optical traps.
  • The findings provide a new tool for nanoparticle assembly research.