Related Experiment Video

Updated: Mar 21, 2026

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

1.3K

The preprotein translocase YidC controls respiratory metabolism in Mycobacterium tuberculosis

Preeti Thakur1,2, Nagavara Prasad Gantasala3, Eira Choudhary1,4

  • 1Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121001 India.

Scientific Reports
|May 12, 2016
PubMed

Insights

Mycobacterium tuberculosis YidC is an essential envelope protein crucial for ATP production and maintaining cellular redox balance. This protein is vital for bacterial respiration and intracellular survival within human macrophages.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • YidC-Oxa1-Alb3 proteins are essential preprotein translocases involved in membrane protein insertion in various organisms.
  • Rv3921c, a putative YidC translocase in Mycobacterium tuberculosis (Mtb), has been identified as essential for bacterial growth in vitro.
  • The precise function of Mtb YidC in mycobacterial pathogens requires further elucidation.

Purpose of the Study:

  • To systematically investigate the function of the YidC protein (Rv3921c) in Mycobacterium tuberculosis.
  • To determine the role of YidC in cellular processes such as ATP production, redox balance, and metabolism.
  • To assess the importance of YidC for Mtb's survival and pathogenesis in host environments.

Main Methods:

  • Characterization of Rv3921c as an envelope protein in Mtb.
  • Analysis of gene expression changes upon Rv3921c depletion, focusing on hypoxic genes, ATP synthases, and metabolic pathways.
  • Proteomic analysis to identify YidC interacting partners, particularly those involved in respiration.
  • Assessment of Mtb's intracellular survival in human macrophages under conditions of YidC modulation.

Main Results:

  • Mtb YidC was confirmed as an envelope protein essential for ATP production and cellular redox balance.
  • Depletion of Rv3921c significantly altered the expression of hypoxic genes, ATP synthases, and key metabolic and respiratory proteins.
  • YidC was found to associate with respiratory complex proteins, including succinate dehydrogenases and ubiquinol-cytochrome C reductase.
  • YidC is indispensable for the intracellular survival of Mtb within human macrophages.

Conclusions:

  • Mtb YidC plays a critical role in regulating respiratory metabolism and maintaining cellular homeostasis.
  • The protein is essential for Mtb's ability to thrive and survive within the host macrophage environment.
  • YidC represents a potential therapeutic target for combating tuberculosis infections.

More Related Videos

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research09:53

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Published on: June 7, 2024

1.7K
Mitochondrial Respiration Quantification in Yeast Whole Cells07:15

Mitochondrial Respiration Quantification in Yeast Whole Cells

Published on: November 8, 2024

1.4K

Related Concept Videos

Respiration Pathways01:26

Respiration Pathways

Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
891
Chemical Factors Affecting Respiration Centers01:31

Chemical Factors Affecting Respiration Centers

Chemical factors such as changing CO2, O2, and H+ levels in arterial blood play a critical role in influencing respiration depth and rates. These variations are detected by chemoreceptors—specialized sensors located in two primary body areas. Central chemoreceptors are found throughout the brain stem, including the ventrolateral medulla, while peripheral chemoreceptors are located in the aortic arch and carotid arteries.
CO2 has a potent influence on respiration and is strictly regulated....
2.7K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
3.1K
Physiological Control of Respiration01:23

Physiological Control of Respiration

Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
6.7K
The Electron Transport Chain01:30

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
21.2K
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
1.2K