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Related Experiment Video

Updated: Oct 23, 2025

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
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Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development.

Jaroslav Icha1, Christopher Schmied2, Jaydeep Sidhaye2

  • 1Max Planck Institute of Molecular Cell Biology and Genetics; icha@mpi-cbg.de.

Journal of Visualized Experiments : Jove
|May 12, 2016
PubMed
Summary
This summary is machine-generated.

Light sheet fluorescence microscopy (LSFM) enables detailed embryonic development imaging with low phototoxicity. This protocol details LSFM use and processing large multiview datasets for developmental biologists.

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Area of Science:

  • Developmental Biology
  • Microscopy Techniques
  • Bioimaging

Background:

  • Light sheet fluorescence microscopy (LSFM) offers advantages like low phototoxicity and fast acquisition for imaging embryonic development.
  • Multiview imaging in LSFM generates large datasets, posing processing challenges.
  • Commercial LSFM systems are now accessible, broadening their use for developmental biologists.

Purpose of the Study:

  • To provide a protocol for using LSFM in developmental biology research.
  • To address the challenges of processing large multiview LSFM datasets.
  • To demonstrate LSFM applications using zebrafish eye development as a model.

Main Methods:

  • Detailed experimental protocol for mounting zebrafish embryos for LSFM.
  • Guidance on imaging using commercially available LSFM systems.
  • Pipeline for registration and fusion of multiview datasets using an open-source Fiji plugin.

Main Results:

  • Demonstration of LSFM for imaging zebrafish eye development across multiple scales.
  • Successful processing of large multiview datasets.
  • A practical protocol adaptable for various LSFM experiments.

Conclusions:

  • LSFM is a powerful tool for developmental biology, especially with accessible commercial systems.
  • Effective processing pipelines are crucial for handling large LSFM datasets.
  • This protocol provides valuable insights and troubleshooting for LSFM users.