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Related Concept Videos

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD22608:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

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Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is...
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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells08:22

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This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid...
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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry09:02

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This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow...
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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue07:38

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In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal...
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Culture of myeloid dendritic cells from bone marrow precursors06:39

Culture of myeloid dendritic cells from bone marrow precursors

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This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are...
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Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

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Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
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Related Experiment Video

Updated: Jan 19, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

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The Innate Lymphoid Cell Precursor.

Isabel E Ishizuka1,2, Michael G Constantinides1,2,3, Herman Gudjonson1,4,5

  • 1Committee on Immunology, The University of Chicago, Illinois 60637;

Annual Review of Immunology
|May 12, 2016
PubMed
Summary
This summary is machine-generated.

Innate lymphoid cells (ILCs) are crucial for mucosal immunity and tissue repair. This review maps their development from early precursors, clarifying lineage relationships and transcription factor networks.

Keywords:
innate lymphoid cell developmentinnate lymphoid cell precursorpromyelocytic leukemia zinc factortranscription factors

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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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Related Experiment Videos

Last Updated: Jan 19, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Tissue-resident innate lymphoid cells (ILCs) orchestrate type 1, 2, and 3 immunity, tissue repair, and immune regulation.
  • The complexity, redundancy, and plasticity of ILC populations raise questions about their precise lineage relationships.

Purpose of the Study:

  • To review advances in mapping the emergence of ILC lineages from early lymphoid precursors.
  • To discuss the lineage relationships of ILCs with natural killer (NK) cells and lymphoid tissue inducer (LTi) cells.
  • To review the transcription factor networks governing ILC development.

Main Methods:

  • Literature review of recent advances in ILC research.
  • Analysis of developmental pathways and transcription factor roles.
  • Comparison of ILC lineages with related immune cells.

Main Results:

  • Identification of a common ILC precursor expressing the transcription factor PLZF.
  • Elucidation of lineage relationships between ILCs, NK cells, and LTi cells.
  • Growing understanding of the transcription factor network directing ILC development.

Conclusions:

  • Mapping ILC emergence clarifies their complex lineage relationships.
  • Understanding ILC development is key to advancing mucosal immunity and allergy research.
  • Transcription factors play a critical role in directing ILC lineage commitment.