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Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

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Updated: Mar 21, 2026

Aortic Ring Assay

Aortic Ring Assay

Published on: November 24, 2009

Human Arterial Ring Angiogenesis Assay.

Giorgio Seano^1,2, Luca Primo^3,4

¹Laboratory of Cell Migration, Candiolo Cancer Institute - FPO, IRCCS, Candiolo, Turin, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2016

Summary

This summary is machine-generated.

The human arterial ring assay models angiogenesis, enabling 3D live studies of capillary formation from umbilical cord artery explants. This innovative ex vivo system aids in identifying genes, screening drugs, and analyzing tumor effects on blood vessel growth.

Keywords:

3D cell culture Drug discovery Human explants Lentiviral transduction Sprouting angiogenesis Tumor microenvironment Umbilical artery VEGF

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Aortic Ring Assay

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Published on: November 24, 2009

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Area of Science:

Biomedical research
Cell biology
Vascular biology

Background:

Angiogenesis is crucial for development and disease.
Existing models often lack the complexity of human physiology.
A need exists for robust ex vivo systems to study human angiogenesis.

Purpose of the Study:

To describe and validate the human arterial ring (hAR) assay.
To showcase the hAR assay's utility in studying angiogenesis.
To highlight applications in drug screening and biomarker discovery.

Main Methods:

Utilizing human umbilical cord artery explants.
Embedding explants in a three-dimensional gel matrix.
Culturing explants to observe and analyze capillary-like structure formation.

Main Results:

The hAR assay successfully recapitulates key steps of angiogenesis.
Demonstrated endothelial sprouting, migration, and differentiation into capillaries.
Established a functional ex vivo model for live, 3D angiogenesis studies.

Conclusions:

The hAR assay is a powerful, innovative tool for studying human angiogenesis.
This model facilitates the identification of angiogenesis regulators and drug targets.
It offers dynamic analysis of tumor microenvironment interactions with vessel formation.