Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia
Randall J Cohrs1,2, Hussain Badani3, Nathan Bos3
1Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA. randall.cohrs@ucdenver.edu.
Journal of Neurovirology
|May 14, 2016
Abstract:
Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological replicates of cells from dissociated randomly distributed human trigeminal ganglia (TG) of four subjects revealed an increase in both parameters and in both viruses during 5 days of culture, with no further change by 10 days. Dissociated TG provides a platform to analyze initiation of latent virus DNA replication within 5 days of culture.
