Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

42.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
42.7K
The DNA Replication Fork01:02

The DNA Replication Fork

20.0K
20.0K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.5K
2.5K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic Impact and Metastasis Propensity of Grade Group 5 Prostate Cancer Following Permanent Seed Implantation Brachytherapy-Based Trimodality Therapy: Implications for Intensified Staging and Systemic Therapy Optimization.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

Validation of virtual non-contrast imaging and related metrics with variable iodine concentrations and flow rates in dual-energy CT: a phantom study.

Fukushima journal of medical science·2026
Same author

FOXO1 Is Required for Growth and Viability of Cancer-Associated Fibroblasts in Human Breast Carcinomas.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

Soft microfingers with flexible tactile sensor using liquid metal for in situ evaluation of cellular spheroid stiffness.

Scientific reports·2026
Same author

Meeting report on FASEB protein arginine methylation: mechanism to therapeutics.

The Journal of biological chemistry·2026
Same author

Identification of stomatin-1 (STO-1) as a novel arginine monomethylated protein in Caenorhabditis elegans.

Bioscience, biotechnology, and biochemistry·2025

Related Experiment Video

Updated: Mar 21, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K

CTF18 interacts with replication protein A in response to replication stress.

Yuta Kaneko1, Hiroaki Daitoku1, Chihiro Komeno1

  • 1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305‑8577, Japan.

Molecular Medicine Reports
|May 14, 2016
PubMed
Summary

The study reveals that the CTF18-RFC complex interacts with RPA during replication stress, a key event for maintaining genome integrity. This interaction is crucial for activating cellular responses to DNA damage and replication defects.

More Related Videos

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

9.6K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

Related Experiment Videos

Last Updated: Mar 21, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K
Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

9.6K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Replication stress response is vital for maintaining genome integrity in eukaryotic cells.
  • The CTF18-RFC complex regulates S-phase checkpoint signaling and translesion DNA synthesis.
  • Mechanisms of CTF18-RFC recruitment to stalled replication forks remain unclear.

Purpose of the Study:

  • To investigate how CTF18-RFC responds to replication stress.
  • To elucidate the recruitment mechanism of CTF18-RFC to stalled replication forks.
  • To understand the molecular basis of CTF18-RFC's role in replication stress response.

Main Methods:

  • In situ proximity ligation assay (PLA) in mammalian cells.
  • Hydroxyurea treatment to induce replication stress during S phase.
  • Ultraviolet irradiation to trigger translesion DNA synthesis.

Main Results:

  • Endogenous CTF18 forms a physical complex with replication protein A (RPA).
  • CTF18-RPA interaction occurs in chromatin upon replication stress (hydroxyurea or UV irradiation).
  • CTF18-RPA interaction kinetics correlate with checkpoint kinase 1 phosphorylation, indicating ATM/ATR pathway activation.

Conclusions:

  • CTF18-RFC interacts with RPA at stalled replication forks during replication stress.
  • This interaction is a critical step in activating replication stress response pathways.
  • Provides novel insights into CTF18-RFC's regulatory role in maintaining genome stability.