Related Experiment Video

Updated: Mar 21, 2026

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K

Optimize the interactions at S4 with efficient inhibitors targeting 3C proteinase from enterovirus 71

Lanjun Zhang1, Guolong Huang1, Qixu Cai1

  • 1State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, State-Province Joint Engineering Laboratory of Targeted Drugs from Natural Products, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.

Journal of Molecular Recognition : JMR
|May 18, 2016
PubMed

Insights

Enterovirus 71 (EV71) causes severe hand, foot, and mouth disease. New potent inhibitors targeting the EV71 3C proteinase were developed, showing promise for antiviral therapies.

Area of Science:

  • Virology
  • Drug Discovery
  • Structural Biology

Background:

  • Enterovirus 71 (EV71) is a major cause of hand, foot, and mouth disease.
  • EV71 infections can lead to severe central nervous system complications.
  • The viral 3C proteinase (3Cpro) is essential for EV71 replication and a key drug target.

Purpose of the Study:

  • To evaluate interactions between EV71 3Cpro and novel inhibitors.
  • To identify structural features that enhance inhibitor potency.
  • To develop new antiviral agents against EV71.

Main Methods:

  • X-ray crystallography to determine inhibitor-protein interactions.
  • Functional assays to assess inhibitor efficacy.
  • Structure-activity relationship analysis to optimize inhibitor design.

Main Results:

  • Key interactions at the S4 binding site were identified as crucial for enhancing inhibitor potency.
  • A new series of potent EV71 3Cpro inhibitors with high ligand efficiency were generated.
  • The findings provide a basis for developing effective antivirals against EV71.

Conclusions:

  • Optimizing substrate binding interactions, particularly at the S4 site, significantly improves inhibitor potency against EV71 3Cpro.
  • The newly developed inhibitors represent promising candidates for antiviral drug development to combat EV71-associated diseases.
  • This research contributes to the ongoing efforts to control and treat severe EV71 infections.
Keywords:
3C proteinasecysteine proteasedrug design and developmentdrug interactionenterovirus 71hand, foot and mouth disease

More Related Videos

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication10:16

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

4.3K
Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds09:29

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

31.0K

Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
1
Pharmacokinetics: Drug–Food and Drug–Viral Interactions01:26

Pharmacokinetics: Drug–Food and Drug–Viral Interactions

A drug interaction occurs when the concurrent use of another drug, food, or an external substance alters the pharmacological activity of a drug. This interaction can modify the action of the original drug, affecting its effectiveness and safety.Drug–food interactions are significant as they impact drug absorption, metabolism, and excretion. For example, grapefruit juice is a well-known disruptor of drug metabolism. It inhibits the cytochrome P450 3A4 enzyme, crucial for the metabolism of...
444
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
94.7K
Dipeptidyl Peptidase 4 Inhibitors01:23

Dipeptidyl Peptidase 4 Inhibitors

Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a...
979