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Published on: March 13, 2015
EFFECT OF STORAGE TIME AND STORAGE CONDITIONS ON ANTIBODY DETECTION IN BLOOD SAMPLES COLLECTED ON FILTER PAPER
Sarah Bevins1, Ryan Pappert2, John Young2
11 US Department of Agriculture, Wildlife Services, National Wildlife Research Center, 4101 Laporte Ave., Fort Collins, Colorado 80521, USA.
Abstract:
Using filter paper to collect blood from wildlife for antibody analysis can be a powerful technique to simplify the collection, transport, and storage of blood samples. Despite these advantages, there are limited data that detail how long these samples can be stored and how storage conditions affect antibody longevity. We used blood samples collected on filter paper from coyotes experimentally infected with Yersinia pestis to determine optimum sample storage conditions over time. Blood samples collected on filter paper were stored for 454 d or more in four groups: 1) at ambient temperature and at ambient relative humidity, 2) at ambient temperature with desiccant, 3) at 4 C with desiccant, and 4) at -20 C with desiccant. Samples stored at 4 C or -20 C with desiccant had detectable antibody for a longer period of time than the samples stored at room temperature.
