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Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Microarray-integrated optoelectrofluidic immunoassay system.

Dongsik Han¹, Je-Kyun Park¹

¹Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST) , 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Biomicrofluidics

|May 19, 2016

Summary

This summary is machine-generated.

This study introduces a novel optoelectrofluidic device to enhance microarray immunoassays. The method significantly reduces reaction times and improves efficiency for detecting target molecules, enabling faster multiplexed protein analysis.

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Area of Science:

Microfluidics
Bioanalytical Chemistry
Optoelectronics

Background:

Microarray-based analytical platforms are crucial in biological assays.
Low reaction efficiency due to slow mass transport (analyte depletion) limits practical applications of microarrays.

Purpose of the Study:

To improve the efficiency of microarray-based immunoassays.
To overcome limitations of slow mass transport in conventional microarray formats.

Main Methods:

Integration of an optoelectrofluidic device with a glass slide-based microarray.
Utilizing optically induced AC electroosmotic flows (OIACEF) under AC voltage.
Employing a microarray-patterned light to actively enhance mass transport.

Main Results:

Reduced reaction time from over 30 minutes to 10 minutes.
Successfully performed heterogeneous immunoassays with a small sample volume (5 μl).
Demonstrated improved efficiency compared to static conditions and enabled multiplexed protein detection.

Conclusions:

Optically induced electrokinetic phenomenon offers a novel approach to enhance microarray immunoassay efficiency.
The integrated system significantly accelerates mass transport and reduces assay times.
This technology holds promise for rapid and efficient multiplexed bioanalysis.