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Studying the Cytoskeleton

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Updated: Mar 21, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Using Fluorescence Microscopy to Study Mitosis.

Sai K Balchand¹, Barbara J Mann¹, Patricia Wadsworth²

¹Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts Amherst, 458 Morrill IV South, Amherst, MA, 01003, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 20, 2016

Summary

This summary is machine-generated.

This study highlights fluorescence microscopy for visualizing cell division (mitosis). It emphasizes minimizing light damage to live cells for clearer images of the mitotic spindle.

Keywords:

Confocal laser scanning microscopy Mitosis Spinning disc confocal microscopy

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Cell Biology
Microscopy
Molecular Biology

Background:

Fluorescence microscopy is crucial for understanding cell division.
Key insights into mitosis rely on visualizing cellular processes.
The mitotic spindle is a primary target for microscopic study.

Purpose of the Study:

To outline essential considerations for imaging mitosis using fluorescence microscopy.
To stress the balance between image quality and cellular health.
To provide guidance for researchers studying the cell cycle.

Main Methods:

Utilizing fluorescence microscopy techniques.
Implementing strategies to reduce phototoxicity.
Optimizing imaging parameters for live-cell studies.

Main Results:

Identified critical factors for successful mitosis imaging.
Demonstrated methods for minimizing light-induced cellular damage.
Achieved informative images of the mitotic spindle with reduced photobleaching and photodamage.

Conclusions:

Effective fluorescence microscopy requires careful optimization.
Minimizing cellular damage is paramount for accurate mitosis studies.
This approach enhances the study of cell division and the mitotic spindle.