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Hepatitis

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.

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Multiplex Reverse Transcriptase-PCR for Simultaneous Detection of Hepatitis B, C, and E Virus.

Gunjan Garg¹, Deepak Kumar², Mohammad Asim¹

¹Department of Medicine, Maulana Azad Medical College, University of Delhi, New Delhi, India.

Journal of Clinical and Experimental Hepatology

|May 20, 2016

Summary

A new multiplex Reverse Transcriptase-PCR assay enables rapid, cost-effective screening for Hepatitis B, C, and E viruses. This method accurately detects single and co-infections, crucial for epidemiological studies and managing liver disease.

Keywords:

ELISA, enzyme linked immunosorbent assay HAV, hepatitis A virus HBV, hepatitis B virus HCV, hepatitis C virus HEV, hepatitis E virus NATs, nucleic acid amplification technologies NPV, negative predictive value PCR, polymerase chain reaction PPV, positive predictive value RT-PCR, Reverse Transcriptase-Polymerase Chain Reaction hepatitis B virus hepatitis C virus hepatitis E virus liver diseases multiplex PCR

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Area of Science:

Virology
Molecular Biology
Hepatology

Background:

Hepatitis B virus (HBV), HCV, and HEV can cause single or co-infections in liver disease patients.
Efficient screening methods are vital for large-scale epidemiological studies of these viral pathogens.

Purpose of the Study:

To develop a multiplex Reverse Transcriptase-PCR (RT-PCR) assay for simultaneous detection of HBV, HCV, and HEV.
To establish a rapid, reliable, and cost-effective screening tool for these hepatitis viruses.

Main Methods:

Serum samples from 54 patients with confirmed HBV, HCV, or HEV infections were tested.
A multiplex RT-PCR assay using specific primers for HBV, HCV, and HEV was developed and validated.
Assay performance was compared to uniplex PCR/RT-PCR, with positive samples confirmed by sequencing.

Main Results:

The multiplex PCR assay demonstrated 100% specificity.
Sensitivity was high: 89% for HBV, 87% for HCV, and 74% for HEV.
RT-multiplex PCR results correlated well with uniplex PCR findings.

Conclusions:

Multiplex RT-PCR offers a simple, reliable, and sensitive method for simultaneous detection of HBV, HCV, and HEV.
This assay is cost-effective for rapid screening, particularly for HEV co-infections in Asian countries contributing to chronic liver disease decompensation.