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Updated: Mar 21, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620
Katarzyna A Duda1, Sandra Petersen2, Otto Holst2
1Junior Research Group Allergobiochemistry.
Abstract:
Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria. LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments. Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids. The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
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