Related Experiment Video
Updated: Mar 21, 2026

11:04
The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
Published on: February 19, 2013
27.2K
Development of a real-time PCR assay for quantification of Citrobacter rodentium
Sofia Sagaidak1, Amel Taibi1, Bijun Wen1
1Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada.
Journal of Microbiological Methods
|May 20, 2016
Abstract:
Molecular tools to quantify Citrobacter rodentium are not available. We developed a quantitative PCR assay targeting the espB gene. This assay is specific, has a linearity range of about 6.7×10(1) to 6.7×10(6)cells/PCR reaction (92% efficiency) and a detection limit of about 10(4)cells/g wet feces.

