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Isolation and primary structure of rat secretin
D Gossen1, A Vandermeers, M C Vandermeers-Piret
1Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
Biochemical and Biophysical Research Communications
|April 28, 1989
Abstract:
A major form of rat secretin was purified to homogeneity from small intestine, being detected with a porcine secretin radioimmunoassay throughout 7 chromatographic steps. The sequence of the heptacosapeptide amide H-S-D-G-T-F-T-S-E-L-S-R-L-Q-D-S-A-R-L-Q-R-L-L-Q-G-L-V-NH2 shows that rat secretin has a glutamine residue in position 14 instead of arginine as in pig secretin.