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Related Experiment Video

Updated: Mar 21, 2026

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

Live single-cell laser tag.

Loïc Binan^1,2, Javier Mazzaferri¹, Karine Choquet^3,4

¹Research Center of the Maisonneuve-Rosemont Hospital, Montreal, Quebec, Canada.

Nature Communications

|May 21, 2016

Summary

This summary is machine-generated.

Cell labelling via photobleaching (CLaP) offers non-invasive, genetic-free cell tagging for diverse applications. This method stably marks individual cells for tracking, genomics, and microengineering, advancing cell biology research.

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Area of Science:

Cell Biology
Biotechnology
Microscopy

Background:

Non-invasive cell tagging is crucial for understanding cellular processes.
Existing methods often rely on genetic engineering, limiting applications.
A need exists for versatile, genetic-free cell labeling techniques.

Purpose of the Study:

To introduce cell labelling via photobleaching (CLaP) as a novel, non-invasive cell tagging method.
To demonstrate CLaP's versatility for various cell biology applications, including genomics and microengineering.
To characterize the stability, toxicity, and inheritance of the CLaP-induced cell mark.

Main Methods:

CLaP utilizes laser illumination to crosslink biotin onto the plasma membrane.
Streptavidin conjugates are used to label biotinylated cells.
The method was combined with microfluidics for single-cell capture and next-generation sequencing.
CLaP was also used to induce transient cell adhesion for microengineering.

Main Results:

CLaP provides instant and specific tagging of individual cells based on morphology, behavior, or position.
The incorporated mark is stable, non-toxic, retained for days, and passed through cell division.
CLaP-labeled cells were successfully used for transcriptome-wide sequencing.
CLaP facilitated the creation of patterned cell cultures through induced adhesion.

Conclusions:

CLaP is a powerful, genetic-free tool for cell labeling with broad applications in cell biology.
The method enables advanced genomic analyses and precise control in cell culture microengineering.
CLaP significantly advances the capabilities for non-invasive cell tracking and manipulation.