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TRIO Platform: A Novel Low Profile In vivo Imaging Support and Restraint System for Mice
Vladislav Voziyanov1, Benjamin S Kemp2, Chelsea A Dressel2
1Integrated Neuroscience and Imaging Lab, Center for Biomedical Engineering and Rehabilitation Sciences, Louisiana Tech UniversityRuston, LA, USA; School of Biological and Health Systems Engineering, Ira A. Fulton School of Engineering, Arizona State UniversityTempe, AZ, USA.
Researchers developed a compact, integrated system for in vivo mouse brain imaging. This system combines head fixation, anesthesia delivery, and warming, simplifying longitudinal studies and improving image quality.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Optical Imaging
Background:
- In vivo optical imaging of the mouse brain requires precise control of anesthesia, temperature, and head stability.
- Current setups often involve multiple, bulky components that are difficult to integrate and position accurately on standard microscope stages.
- Maintaining consistent animal positioning is crucial for longitudinal studies, especially with deep brain imaging using gradient index (GRIN) lenses.
Purpose of the Study:
- To design and validate a compact, integrated system for in vivo optical imaging in mice.
- To overcome the challenges associated with using multiple separate systems for anesthesia, temperature control, and head fixation.
- To improve the ease and accuracy of longitudinal imaging studies by ensuring reproducible animal positioning.
Main Methods:
- Development of a THREE-IN-ONE (TRIO) Platform integrating head fixation, gas anesthesia mask, and warm water heating.
- Utilizing a compact, low-profile design (20 mm above stage) suitable for various upright microscopes.
- Fabrication of components using 3D printing based on SOLIDWORKS® designs.
Main Results:
- The TRIO Platform successfully integrated head fixation, anesthesia delivery, and warming in a single unit.
- The system demonstrated a small footprint and low profile, facilitating use with diverse microscope setups.
- Over 40 imaging sessions using isoflurane (average 2 h each) were completed without leaks or malfunctions.
- The system's design allows for easy modification for other small animal models.
Conclusions:
- The TRIO Platform offers a streamlined solution for in vivo mouse brain optical imaging.
- This integrated system simplifies experimental setup, enhances animal stability, and ensures precise positioning for longitudinal studies.
- 3D printing enables cost-effective fabrication and customization of the imaging support system.
