Related Experiment Video

Updated: Mar 20, 2026

Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

14.7K

Identification of a Peptide Produced by Bifidobacterium longum CECT 7210 with Antirotaviral Activity

Empar Chenoll1, Beatriz Casinos1, Esther Bataller1

  • 1Department of AgroFood Biotechnology, Biópolis S.L. Valencia, Spain.

Frontiers in Microbiology
|May 21, 2016
PubMed

Insights

A probiotic bacterium, Bifidobacterium longum subsp. infantis CECT 7210, produces an 11-amino acid peptide that inhibits rotavirus replication. This peptide, identified as 11-mer, shows potential for enhancing rotavirus vaccine efficacy.

Area of Science:

  • Microbiology
  • Virology
  • Gastroenterology

Background:

  • Rotavirus is a primary cause of infant diarrhea and enteritis.
  • Probiotics are being investigated to boost rotavirus vaccine effectiveness.
  • Bifidobacterium longum subsp. infantis CECT 7210 previously showed in vitro and in vivo rotavirus inhibition.

Purpose of the Study:

  • To systematically identify the molecule from B. longum subsp. infantis CECT 7210 responsible for rotavirus inhibition.
  • To characterize the inhibitory molecule and its mechanism of action.

Main Methods:

  • Supernatant protease digestion to determine the nature of the active substance.
  • Cationic exchange chromatography for purification of the inhibitory compound.
  • Synthesis and functional verification of the identified peptide against rotavirus strains in cell lines.
  • Detection of protease activity in bacterial supernatant.

Main Results:

  • The rotavirus inhibitory substance is proteinaceous and released into the supernatant.
  • An 11-amino acid peptide (11-mer peptide, MHQPHQPLPPT) was identified as the active molecule.
  • The synthesized 11-mer peptide inhibited replication of Wa, Ito, and VA70 rotavirus strains in HT-29 and MA-104 cells.
  • Protease activity was detected in the bacterial supernatant, correlating with 11-mer peptide release.

Conclusions:

  • The 11-mer peptide produced by B. longum subsp. infantis CECT 7210 directly inhibits rotavirus replication.
  • This peptide represents a potential therapeutic or adjuvant agent for improving rotavirus vaccine outcomes.
  • Further research into the specific protease involved is warranted.

Keywords:
11-mer peptideB. longum subsp. infantis CECT 7210probioticsproteaserotavirus

More Related Videos

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors10:29

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors

Published on: May 9, 2025

2.6K
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.5K

Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
1
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
15
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.8K