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Related Concept Videos

Gap Junctions01:37

Gap Junctions

58.2K
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
58.2K
Patch Clamp01:18

Patch Clamp

7.4K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
7.4K

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Related Experiment Video

Updated: Mar 20, 2026

Single-cell Microinjection for Cell Communication Analysis
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Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

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Microinjection Technique for Assessment of Gap Junction Function.

Michael D Fridman1, Jun Liu2, Yu Sun2

  • 1Department of Physiology and Experimental Medicine, Hospital for Sick Children, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2016
PubMed
Summary
This summary is machine-generated.

Microinjection is a valuable technique for assessing gap junctions, crucial for cell communication and tissue function. Automation advancements are enhancing its precision and success rates for research and therapeutic development.

Keywords:
Cellular communicationConnexinDye transferGap junctionMicroinjection

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Area of Science:

  • Cellular Biology
  • Biophysics

Background:

  • Gap junctions are vital for mammalian tissue function, including neurons and cardiomyocytes.
  • Understanding gap junction function is key to disease pathophysiology and therapeutic development.
  • Microinjection offers detailed cellular analysis, surpassing simpler methods.

Purpose of the Study:

  • To describe the fundamental microinjection technique.
  • To highlight its utility in assessing gap junctions.
  • To discuss automation's role in improving microinjection.

Main Methods:

  • Detailed description of the microinjection process.
  • Discussion of cell isolation and kinetic quantification.
  • Exploration of automation technologies for microinjection.

Main Results:

  • Microinjection provides superior data for cell isolation and kinetic analysis.
  • The technique allows for cross-comparison across multiple cell lines.
  • Automation shows promise in increasing microinjection success rates.

Conclusions:

  • Microinjection is an indispensable tool for gap junction research.
  • Despite challenges, its benefits are significant for cellular and tissue studies.
  • Emerging automation technologies are poised to further enhance this technique's capabilities.