Related Experiment Video
Updated: Mar 20, 2026
08:29
Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
Published on: February 6, 2015
24.6K
Novel liquid bubble dissection technique for DMEK lenticule preparation
Peter Szurman1,2, Kai Januschowski3,4, Annekatrin Rickmann3
1Knappschaft Eye Clinic Sulzbach, Knappschaft Hospital Saar, An der Klinik 10, 66280, Sulzbach/Saar, Germany. Peter.Szurman@kksaar.de.
Summary
A new liquid bubble dissection technique simplifies Descemet membrane (DM) preparation for Descemet endothelial keratoplasty (DMEK). This rapid, reproducible method minimizes tissue manipulation and achieves a high success rate for DMEK lenticule preparation.
Area of Science:
- Ophthalmology
- Corneal Surgery
- Tissue Engineering
Background:
- Descemet endothelial keratoplasty (DMEK) is a preferred procedure for endothelial cell disorders.
- No-touch injection cartridges have improved DMEK, but delicate Descemet's membrane (DM) preparation remains challenging.
Purpose of the Study:
- To introduce and evaluate a novel liquid bubble dissection technique for preparing the Descemet's membrane (DM) for DMEK.
- To assess the advantages of this technique in terms of speed, reproducibility, and graft integrity.
Main Methods:
- A novel liquid bubble dissection technique was developed for DM preparation.
- The technique involves sharp and blunt dissection to create a tunnel, followed by liquid bubble dissection for complete detachment.
- Performed on 86 DMEK lenticule preparations between February and September 2015.
Main Results:
- The liquid bubble dissection technique detached the DM in approximately 3 minutes (mean 194 ± 20 seconds).
- 92% of preparations were uncomplicated, with a 99% overall success rate.
- The graft failure rate was 1.16%, comparable to existing methods.
Conclusions:
- The liquid bubble technique is easy to learn, rapid, and highly reproducible.
- It requires minimal tissue manipulation ('no touch') and no special equipment.
- The technique offers a low graft preparation failure rate and allows selective staining, avoiding direct contact.
