Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Proteomic profiling of whole tissue sections in cardiac ATTR amyloidosis reveals increased extracellular matrix remodeling.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026

Same author

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities.

The Plant cell·2026

Same author

Integrated multi-omics identifies distinct macrophage alterations during progression of metabolic dysfunction-associated steatohepatitis.

Nature genetics·2026

Same author

Confirmation of monotypic immunofluorescence staining by mass spectrometry in a case of proliferative glomerulonephritis.

Histopathology·2026

Same author

Transcriptomic Signatures of Nitrate Response in Rapeseed Genotypes With Distinct Root System Sizes.

Physiologia plantarum·2026

Same author

FoldDelay web server: an online tool to quantify translation-driven delays in protein native contact formation.

Nucleic acids research·2026

Same journal

From wrinkled seeds to plant oil accumulation networks: The legacy of a Plant Physiology classic.

Plant physiology·2026

Same journal

LcHXK1 mediates glucose signaling to inhibit fruit abscission by phosphorylating LcWRKY42, a feedback regulator in lignin polymerization.

Plant physiology·2026

Same journal

Partial submergence-induced adventitious root emergence in cucumber requires CsRBOHB-mediated ROS production.

Plant physiology·2026

Same journal

JA differentially regulates a SmWLIM1/MYB62-SUS1 module to control male fertility via starch biosynthesis.

Plant physiology·2026

Same journal

The chloroplastic NFU1 maturation factor sustains iron-sulfur cluster assembly in the dark in Chlamydomonas.

Plant physiology·2026

Same journal

Systems-level proteomic models of cotton fiber development: a high-resolution data resource to analyze cell dynamics and trait engineering.

Plant physiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 20, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Sequence-Specific Protein Aggregation Generates Defined Protein Knockdowns in Plants.

Camilla Betti¹, Isabelle Vanhoutte¹, Silvie Coutuer¹

¹Department of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).

Plant Physiology

|May 22, 2016

Summary

This summary is machine-generated.

Scientists harnessed protein aggregation-prone regions (APRs) to selectively reduce specific plant proteins. This method targets proteins involved in brassinosteroid signaling and starch production, offering a new tool for crop improvement.

More Related Videos

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Related Experiment Videos

Last Updated: Mar 20, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Area of Science:

Plant molecular biology
Biochemistry
Crop science

Background:

Protein aggregation is driven by short aggregation-prone regions (APRs) that form specific beta-structured inclusions.
Targeting protein function is crucial for understanding plant development and for agricultural applications.

Purpose of the Study:

To investigate if the sequence specificity of APRs can be used to selectively knock down protein functions in plants.
To explore the potential of induced targeted aggregation for generating beneficial traits in crops.

Main Methods:

Designed synthetic aggregation-prone peptides from APRs of brassinosteroid signaling regulators and starch-degrading enzymes.
Expressed these synthetic APRs in model plants Arabidopsis thaliana and maize (Zea mays).

Main Results:

Stable expression of synthetic APRs induced aggregation of target proteins in plants.
Induced aggregation led to constitutive brassinosteroid responses and increased starch content in Arabidopsis and maize, respectively.
Demonstrated targeted protein knockdown in different subcellular compartments.

Conclusions:

The sequence specificity of APRs can be exploited for targeted protein knockdown in plants.
Induced targeted aggregation is a promising tool for generating beneficial traits in crops, such as enhanced growth and yield.