Postnatal ontogeny of enkephalin fibers in spinal sympathetic nuclei

The postnatal distribution of enkephalin (Enk) fibers is described in preganglionic containing sympathetic nuclei in the rat thoracolumbar spinal cord. In high thoracic spinal cord, at birth, Enk fibers are present in moderate numbers in the intermediolateralis nucleus, pars principalis (ILp), and nucleus intercalatus spinalis (IC). Enkephalin fibers first appear in the dorsal commissural nucleus (dcn) on postnatal day 2. Postnatal day 6 represents a pivotal timepoint when the basic Enk innervation pattern is established. From postnatal day 11 through day 20 there is a gradual accumulation of Enk fibers within each of the sympathetic nuclei such that the density and distribution of immunoreactive fibers approaches the adult appearance by postnatal day 20. An adult pattern is achieved by postnatal day 30. There is a rostral-caudal gradient in the developmental appearance of Enk fibers in sympathetic nuclei such that the ILp nucleus contains Enk fibers on postnatal day 0 in the high thoracic spinal levels compared to postnatal day 6 in low thoracic-high lumbar spinal levels. Examination of the location of Enk fibers during ontogeny highlighted several additional features of the distribution of Enk fibers in the adult animal. Enkephalin fibers delineate two subdivisions of the IC nucleus; a thin dense core of Enk fibers contained within a broader band of moderate numbers of Enk fibers. We also report variations in the general overall pattern of the Enk fiber distribution in high thoracic, middle thoracic, and lwo thoracic-high lumbar spinal cord levels.