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Updated: Mar 20, 2026
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux
Chih-Yang Huang1,2,3, Wei-Jen Ting2, Chih-Yang Huang4
1Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
Background:
Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease (CVD).
Objective:
This study investigated the protective effects of resveratrol against hydrogen peroxide-induced damage in cardiomyocytes.
Design:
In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts were studied at an increasing concentration from 0 to 100 µM.
Results:
Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h of administration.
Conclusions:
These results suggest that resveratrol-regulated autophagy may play a role in degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a possible mechanism by which resveratrol protects the heart during CVD.
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