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Updated: Mar 20, 2026
Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
Published on: July 13, 2010
Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin
Mitsuru Hattori1, Genki Kawamura1, Ryosuke Kojima1
1Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
Abstract:
Fluorescence imaging can elucidate morphological organization and coordinal networks, but its background luminescence degrades the image contrast. Our confocal bioluminescence imaging system uses a luciferase caged substrate, with light passing through multipinhole arrays, causing bioluminescence at a focal plane. After a charge-coupled device camera captures luminescence, the imaging system acquires confocal images of multilayered cells with depth information, supporting quantitative analysis of spatial cellular localization in living tissues.
